High spontaneous mutation frequency in shuttle vector sequences recovered from mammalian cellular DNA.
نویسندگان
چکیده
منابع مشابه
High mutation frequency in DNA transfected into mammalian cells.
The lacI gene of Escherichia coli was used to score mutation in mammalian cells of simian virus 40-based recombinant DNA vectors that provide for replication and selection in both bacterial and mammalian cells. Plasmid DNA was introduced into COS7 simian cells by DEAE-dextran transfection, allowed to replicate in the mammalian cells, and then returned to E. coli for analysis. Mutants in lacI we...
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In mammalian cells, little is known about the nature of recombination-prone regions of the genome. Previously, we reported that the immunoglobulin heavy chain (IgH) mu locus behaved as a hotspot for mitotic, intrachromosomal gene conversion (GC) between repeated mu constant (Cmu) regions in mouse hybridoma cells. To investigate whether elements within the mu gene regulatory region were required...
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In order to study mutagenesis in mammalian cells, stable mouse L-cell lines were established with multiple copies of a lambda phage vector that contains the supF gene of Escherichia coli as a target for mutagenesis. Rescue of viable phage from high molecular weight mouse cell DNA using lambda in vitro packaging extracts was efficient (5 phage per microgram of cell DNA per copy) and yielded a ne...
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The cyanobacteria are an ancient group of Gram-negative prokaryotes. In addition to photosynthesis, the cyanobacteria possess many other interesting features: a) nitrogen-fixation, b) cellular differentiation, c) regulated genomic rearrangements (1, 2) and, d) introns (3). Studies of these features in the filamentous cyanobacteria are hampered by a lack of shuttle vectors. Currently, there is o...
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We previously isolated two human L1 elements (L1.2 and LRE2) as the progenitors of disease-producing insertions. Here, we show these elements can actively retrotranspose in cultured mammalian cells. When stably expressed from an episome in HeLa cells, both elements retrotransposed into a variety of chromosomal locations at a high frequency. The retrotransposed products resembled endogenous L1 i...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1984
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.4.11.2266